Structural characterization of alpha-galactosylated O-glycans from miniature pig kidney and endothelial cells

초록

O-Glycan is a major oligosaccharide expressed on the surface of eukaryotic cells that plays a role in immune responses via cell-protein and cell-cell interaction. Unlike N-glycan and glycolipid-derived glycan, O-glycans have not been previously reported in pig organs. Here, we report the first instance of the identification of alpha 1,3 galactosylated O-glycans from pig kidney and endothelial cells by using MALDI-TOF MS and nano-ESI MS/MS. The MALDI profile showed 28 O-glycans from miniature pig kidney and 18 O-glycans from pig endothelial cells. Among them, three potential alpha-galactosylated glycans considered as immunogenic antigens were identified by CID using ESI-MS/MS; Hex alpha 1,3Hex-HexNAc-HexNAc, Hex alpha 1,3-Hex-Hex-HexNAc-HexNAc, and Hex alpha 1,3Hex-Hex-Hex-Hex-HexNAc. This structural information on glycans will provide significant information to clinical studies regarding immune rejection responses induced by carbohydrate antigens during xenotransplantation. (C) 2013 Elsevier Ltd. All rights reserved.

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