고속 염기서열 분석법을 이용한 자동차 공조 시스템(HAVC systems)의 악취 연관 곰팡이 군집 특성

초록

The Automobile HVAC system is a habitat for odor-associated fungal communities. We investigated the odorassociatedfungal community in an automobile HVAC system using a high-throughput DNA sequencing method. The fungal community structure was evaluated via metagenome analysis. At the phylum level, Ascomycota andBasidiomycota were detected, accounting for 43.41% and 56.49% of the fungal community in the HVAC system,respectively. Columnosphaeria (8.31%), Didymella (5.60%), Davidiella (5.50%), Microxyphium (4.24%),unclassified Pleosporales (2.90%), and Cladosporium (2.79%) were abundant at phylum of Ascomycota andChristiansenia (36.72%), Rhodotorula (10.48%), and Sporidiobolus (2.34%) were abundant at phylum ofBasidiomycota. A total of 22 genera of fungi were isolated and identified from the evaporators of the HVACsystems which support fungal growth and biofilm formation. Among them, Cladosporium, Penicillium, Aspergillus,and Alternaria are the most representative odor-associated fungi in HVAC systems. They were reported to formbiofilm on the surface of HVAC systems with other bacteria by hypha. In addition, they produce various mVOCssuch as 3-methyl-1-butanol, acetic acid, butanoic acid, and methyl isobutyl ketone. Our findings may be usefulfor extending the understanding of odor-associated fungal communities in automobile HVAC systems.

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